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1/20/2023 0 Comments Microsynth latice![]() ![]() So I've built and played this deck, ideally you want to use Chromatic Lantern like effects so when you go to cast other peoples stuff you've got any mana color you need, some of which are Chromatic Orrery, Mycosynth Lattice Celestial Dawn, Smothering Tithe, etc. ![]() Your Deck is Mine (unfinished, help appreciated) 5 days ago works pretty well in my own Karn-deck and is not dependant on Karn himself or the mana rock being around for more than the current turn (summoning sickness when turned into a creature). I am also missing a mana-sink for your endless mana combo(s), so how about Myr Matrix?Īnd you could add even more endless mana combos: Basalt Monolith + Rings of Brighthearth The Lattice is a real gimmick, as anyone even thinking of casting a mass artifact removal would reset the whole board (except your indestructible stuff, which might be your entire board with the Forge), so most won't do it (or get shafted in the next 100+ games in your playgroup).Īnd the Disk just comboes with Forge/Unwinding for a "board wipe" every turn for anyone but you. Monument boosting your mana production, as it adds to ANY source tapping for it (which kinda everything in this deck does) and enabling more mana rocks to combo with your Voltaic Construct. ![]() I am missing Forsaken Monument as well as Mycosynth Lattice and Nevinyrral's Disk ![]() 1/20/2023 0 Comments Imagej colony counting![]() ![]() Although a number of such systems exist, they all focus on enumerating colonies and not on analysing the colony size. Such analyses are largely facilitated by computerised image analysis systems. Moreover, it is often interesting to quantify the size of individual colonies. In large experimental setups, counting of colonies by eye is tiresome and prone to bias. The app can also automatically average a count across a set of plates.The clonogenic cell survival assay is a basic method to study the cytotoxic effect of radiation and chemical toxins. You can then refine the count by marking missed colonies or masking out false positives. Take a picture of your plate and the app will automatically count the number of spots. This handy app costs only $3.99 and is simple to use. Promega’s Colony Counter AppĪs if your iPhone hasn’t already become an indispensable part of your personal life, it can now be used for colony counting thanks to the Colony Counter App developed by Promega and available at the iTunes store ( ). One word of caution: automatic counters can be led astray by imperfect agar, debris, unusual colonies or plaques or even writing on the plate, so it is always wise to validate the system. Basic models just count spots, while fancier models with high-res color cameras can count spots, measure zones of inhibition and provide information on size and shape of the spot. Automatic counters can range in cost from $4200-15,000. If you are faced with hundreds of plates to count each day, an automatic counter can make your life a lot easier. Once an appropriate image of the plate is captured, free software, (such as MATLAB which is available at can be used to identify and count circular spots. Other labs have used flatbed scanners to image plates. Essentially you only need something to hold the plates, a camera, a light source and a computer. If you are mechanically-inclined, you can put together your own automatic counter, as described by Brugger et al. For a small investment (To increase accuracy, count the plate, rotate 90 degrees and count again or add a second person to confirm each plate’s tally.Use a handheld clicker-counter to help you keep track.Use a sharp-tipped marker to mark each colony/plaque as you count it.Divide the plate into grids for ease of counting.Putting the plates on a light box can be very helpful. Sit in a comfortable seat with lighting that illuminates the colonies/plaques the best.If you are counting manually, make life a littler easier for yourself: If you can’t predict the results of your experiment, plate several different dilutions- it will save you time in the end. Likewise, having only one colony/plaque to count is meaningless. For plaques, make sure you have a nice even monolayer of cells with no clumps.Įven with the aid of technology it’s impossible to count hundreds of colonies/plaques in one dish. Make sure your agar plates are smooth and free of bubbles. It may seem silly, but starting with nice plates can make all the difference in an accurate count, whether being done by human or machine. Below is a list of tips and a summary of available technologies for making sure you get accurate results efficiently. Whichever way you approach colony/plaque counting, we are here to help. However, sometimes you are faced with mountains of plates to count and throwing some money and technology at the assay can significantly increase your speed (and save your poor eyes!). It can also be one of the cheapest when performed manually. …but a simple oneĪ good thing about colony counting is that it is probably one of the simplest assays you will ever perform. Necessary because many experiments have an endpoint that requires determining the number of bugs present in a sample evil because it can be tedious work that strains the eyes. I consider colony/plaque counting one of the necessary evils of working with microorganisms. Have you ever emerged from the lab, bleary-eyed, blinking dazedly at the sun after spending hours hunched over a lab bench counting endless bacterial colonies or viral plaques? A necessary evil… ![]() 1/20/2023 0 Comments R markdown plus or minus![]() ![]() Typically in writing for research we need to include special symbols that are not part of the common character set. Special characters for scientific writing This can be useful when the author is already mentioned in the text: Smith says blah can also write an in-text citation, as follows: says says blah. 33-35, 38-39 and *passim*].īlah blah minus sign (-) before the will suppress mention of the author in the citation. r2symbols R package allows the direct insertion of symbols into Rmarkdown and Shiny. Here are some examples from RStudio: Blah blah. Each citation must have a key, composed of + the citation identifier from the database, and may optionally have a prefix, a locator, and a suffix. Knitr::kable(table_3, caption = "Descriptive statistics of symmetry values for each MIS")Ĭitations go inside square brackets and are separated by semicolons. Now add a chunk of code to your paper.Rmd that looks exactly like this: ``` Then upload to analysis/data/raw-data, it will unzip into two files. You need to save this to your computer (click to download): We are going to do live-coding demonstration of how to import data into R in our paper.Rmd. I have slightly modified the code from the original to use in this workshop: Hoggard_et_al_2019_BM.R Code chunks, in-line code The authors have made their materials online at. The Application of Elliptic Fourier Analysis in Understanding Biface Shape and Symmetry Through the British Acheulean. The data includes the atmospheric concentrations of CO2 in parts per million. We will use the co2 data already found in R. ![]() In this tutorial, we will examine how mathematical expressions can be included into R graphics. ![]() Note2: Below I have used links to images on the web but you can very well use an offline image by adding the complete filename (plus the file path if it is in a different directory other then the Jupyter Notebook).For these activities we will use code and data from this excellent article: Specifically, mathematical symbols or entire equations may be needed when building plots. Note1: You can also Drag and Drop your images to the Markdown cell to attach it to the notebook. You can attach graphics (such as images) to a notebook in Markdown cells. On the other hand, code cells allow you to write and run program code like Python. Here’s where you can explain and document the processes. Markdown Cells allows you to write and render Markdown syntax. Recall that a Jupyter Notebook is a series of cells that can store text or code. It is simple to use and helps you to create and share documents that include code, visualizations, and narration. You don’t need to preface it or delimit it to indicate that you are switching from Markdown to HTML - you just use the tags.Īmong the data enthusiasts, Jupyter notebook is in trend. You simply use HTML for any Markup that is not covered by the Markdown syntax. Therefore, the formatting syntax of Markdown tackles just issues that can be expressed in plain text. HTML is a format for publishing, while Markdown is a format for reading. Markdown’s idea is to make reading, writing, and editing prose easy without the intention to create a syntax that’s just for quickly adding HTML tags. Its syntax is minimal, correlating only to a tiny proportion of HTML tags. Nevertheless, Markdown is not a substitute for, or even close to, HTML. Readability, however, is emphasized above all else. 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On her way through, Mary manages to grab on to Zinnia and pull her in with her. Nick and the Ripper arrange to meet Mary at a vortex in Graceland, and Zinnia uses her abilities to push Mary into an opening that takes her into the real world. However later on in the book he captures her and he tells her he loves her and she kisses him and he lets her go.īy the end of the book, The Ripper, Allie, and Nick join forces to defeat the evil Mary Hightower and foil her plan to kill all the world's children in order to bring them into Everlost. Milos and Allie are skinjacking two teenage couples and Milos kisses Allie who later tells him no but Mikey sees and enraged at Milos and Allie leaves and turns back into a monster again. Allie and Mikey part ways with the skinjackers, who Mary later joins forces with. Mikey grows jealous of Milos's relationship with Allie. Mikey discovers that, though he is no longer a monster, he retains the ability to morph himself into monstrous creations of his own mind. Allie's body lies in a comatose state waiting for her soul to rejoin it, and she has the choice whether to remain a skinjacker in Everlost or return to her living body. Allie is tempted by the seductive thrill of skinjacking as she masters the skill, until she discovers the shocking truth about skinjackers: skinjackers are not necessarily dead or alive. Along the way, Allie meets three other skinjackers, led by Milos. Meanwhile, together with Mikey, Allie the Outcast travels home to look for her parents and her own life. ![]() With her abilities, Nick begins to devise a plan to confront and defeat Mary. Zinnia has the ability to "rip," breaking the barrier between the living world and Everlost to pull or push things through. Nick follows the rumor of "Zack the Ripper", revealed to be Zinnia, a girl who died in the Civil War while pretending to be a boy. Due to the rumors Mary has spread about the Chocolate Ogre Nick is being overwhelmed by the memory of the chocolate stain on his face, and he is unable to stop the chocolate from spreading over his body. Nick, in control of a train, is heading south to gather his own support against Mary. Traveling in the memory of the Hindenburg, Mary spreads her propaganda and attracts Afterlights to her cause at a frightening speed. Allie sets off with Mikey, who was once the terrifying monster, McGill, in the book Everlost, to find her parents in hopes that she might put her spirit to rest meanwhile, Nick finds himself in a race against time to save the Afterlights from endless entrapment in Everlost by the Sky Witch, Mary Hightower. Everwild continues the stories of Allie and Nick as they pursue separate goals. ![]() 1/19/2023 0 Comments Dead or alive 6 tv tropes![]() ![]() ![]() Tag team mode of the previous games isn't featured due to the team focusing all its resources on one vs one modes. A single-player campaign, which was described as "cohesive" by director and producer Yohei Shimbori, was developed so that the game can appeal to both casual gamers and the Western audience. The studio announced they sought to slightly tone down the sexualized portrayal of female characters and focused on being an esports title. The game is using a new graphics engine, which in its July update was revised with the help from Sega's internal and well known studio, Sega AM2 who also developed its prequel, Dead or Alive 5. Team Ninja began full-time development on Dead or Alive 6 in December 2017 and publicly revealed it in June 2018. All characters have an attack that does increased damage if done up close to a prone opponent. The juggle Ground Bounce system is also enhanced for the game, allowing players to bound their opponent on the ground for a finisher, with different inputs depending on character. The system in use will always be displayed under the timer. The game retains the 4-point hold system which has been standard since Dead or Alive 2 Ultimate, while including the option for a 3-point hold as in Dead or Alive 2, Dead or Alive 3, and Dead or Alive Dimensions. Can also be cancelled into a Break Blow cancel by pressing H upon successful hit, which allows the player to use the fatal stun status to pursue more combo damage. Break Blow ( ): A powerful strike that goes through the opponent's attack.Break Hold ( ): A hold that can be used against any type of strike: high, mid, or low.Requires 25% of the meter as of version 1.05. Side Attack ( while or ): A quick attack that can be done while side-stepping.Some characters have special follow ups from fatal rushes, such as being able to feint into stances, or even cancelling into standard attacks. If the meter is full, the combo will finish with a Break Blow. Fatal Rush ( ): A chain of up to four high strikes that will put the opponent into a Fatal Stun.It can be used in various ways with the button (or ): New is the Break Gauge which sits under the life bar, starting the first round half-full. The Power Blow and Power Launcher from the previous game have been removed. …It’s not over yet."Ĭharacters See also: List of Dead or Alive 6 characters The passing days are about to be unduly overturned due to an open desire.Īs Kasumi writes to her mother, her pen overflows with hesitation. A so-called genius chuckles at a mysterious light emitting an aura…īehind a quiet time, a sinister plan is set in motion. Meanwhile, Helena Douglas, president of the new DOATEC, is involved in an incident… The sudden of an individual with special powers sent shivers down her spine…Īnd yet another…. " After a sinister incident in a village… Kasumi, a successor of the legendary Mugen Tenshin ninja clan, abandoned her clan and became a “ runaway ninja,” secretly living in a hermitage in a mountain village. ![]() |
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